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E2F proteins bind the NPAT promoter in vivo. Chromatin immunoprecipitation analysis was performed with U2OS cell extracts by using the indicated antibodies. Immunoprecipitated promoter fragments were analyzed by quantitative PCR to determine the relative presence of E2F1 and E2F4 on the indicated promoters as described in Materials and Methods. All results are representative of at least two independent immunoprecipitations and are reported as means and standard deviations.
