FIG. 9.

Effects of inhibition of NPAT expression on cell cycle progression. (A) U2OS cells were transfected with control vector pBS/U6 or pBS/U6/NPAT. At the indicated time posttransfection, cells were harvested and the cell cycle distributions of the transfected cells were analyzed by FACS as described in Materials and Methods. The data represent the results from four independent experiments. (B) Inhibition of NPAT expression attenuates G₁- and S-phase progression. The percentages of transfected cells that had moved out of G₁ or S phase at 48 to 53 h posttransfection were calculated from the data presented in Table 1 as described in Materials and Methods.