TABLE 1.
Effect of inhibition of NPAT expression on G1- and S-phase progressiona
| Phase | % of cells at the following h after transfection with the indicated plasmid:
|
|||||||
|---|---|---|---|---|---|---|---|---|
| 48
|
55
|
61
|
72
|
|||||
| U6 | U6/NPAT | U6 | U6/NPAT | U6 | U6/NPAT | U6 | U6/NPAT | |
| G1 | 35.4 | 22.9 | 13.4 | 15.7 | 10.7 | 10.1 | 4.4 | 5.8 |
| S | 43.3 | 30.1 | 52.1 | 35.0 | 10.9 | 24.3 | 3.6 | 10.8 |
| G2/M | 21.3 | 46.9 | 34.5 | 49.4 | 78.4 | 65.7 | 92.0 | 83.5 |
a
U2OS cells were transfected with pBS/(U6) or pBS/U6/NPAT (U6/NPAT) as described in Materials and Methods. Nocodazole (50 ng/ml) was added to the culture medium 48 h after transfection. Cells were harvested at the indicated times posttransfection, and the distribution of the transfected cells in the cell cycle was analyzed by FACS. The mean results from two independent experiments are shown.