FIG. 5.

In vitro calcification of isolated chondrocytes from wild-type (A, C, E, and G) and annexin A5-deficient (B, D, F, and H) mice. (A and B) Chondrocytes were induced for 8 days in medium supplemented with 10 mM glycerophosphate, 10 mM CaCl₂, and 50 μg of ascorbate/ml; stained for mineral deposits with 0.5% alizarin red; and cleared with 2% potassium hydroxide. (C and D) Higher magnifications of panels A and B, respectively, are shown. Bar, 250 μm. (E and F) Single cell clusters at higher magnifications of panels C and D, respectively, are shown. Bar, 50 μm. (G and H) Chondrocytes cultured in medium without induction are shown.