FIG. 2.

Comparison of JNK expression and activation in WT and Jnk^−/− fibroblasts. JNK and activated JNK (Phospho-JNK) were examined by immunofluorescence microscopy. The cells were treated without (A) and with (B) TNF-α (10 ng/ml for 15 min), fixed, and stained with antibodies to JNK (FITC [green]) and phospho-JNK (Texas red [red]). DNA was stained with DAPI (blue). The cells were imaged by conventional fluorescence microscopy.