FIG. 2.

A second ASAP complex containing Acinus-S. (a) Silver stain of an SDS-4 to 15% (wt/vol) polyacrylamide gradient gel containing aliquots of the indicated fractions derived from the final MonoP column. Components of the ASAP-S complex are indicated at the right side of the panel. A putative alternatively phosphorylated or partially degraded form of RNPS1 is marked with an asterisk. HMW, higher-molecular-weight bands. (b) Western blot analysis of fraction 33 of the MonoP column (lanes 2, 4, 6, 8, and 10). The Acinus antibodies used in the Western blot are indicated at the top (compare with Fig. 1a). High-molecular-mass protein standards [M] (lanes 1, 3, 5, 7, and 9) are indicated at the left.