FIG. 2.

Physical associations between Eco1p and the small subunits of RFC DNA replication factors. Extracts from cells harboring either Rfc2-FLAGp (Rfc2p), Rfc3-FLAGp (Rfc3p), Rfc4-FLAGp (Rfc4p), or Rfc5-FLAGp (Rfc5p) were tested for association with bead matrices as described in the text. Each RFC small subunit interacted specifically with GST-Eco1p coupled to glutathione Sepharose beads but not to glutathione Sepharose beads alone (beads) or coupled to GST (GST). In contrast, FLAG-tagged bacterial alkaline phosphatase (BAP) did not bind to either beads coupled to GST or GST-Eco1p. See text for details.