FIG. 2.

Subcellular localization of CrkL in migrating cells. (A to C) Subcellular localization of endogenous CrkL, paxillin, and F-actin. NIH 3T3 cells were plated on glass coverslips coated with fibronectin. Photographs of migrating cells were taken 3 h after generation of the cell-free zone. (A) The upper row shows quiescent NIH 3T3 cells; the lower row shows migrating NIH 3T3 cells. (B) The panel shows focal adhesion localization of CrkL in a large subset of migrating NIH 3T3 cells. (C) A higher magnification of the part of the cell highlighted by a white rectangle in panel B. The images are merged for three components: F-actin, CrkL, and paxillin. The panel at the right shows enlarged focal adhesion structures, where whiteness indicates overlap among F-actin, CrkL, and paxillin.