FIG. 3.

Subcellular targeting of CRKL to focal adhesions rescues motility defects of SYF cells. (A) The panel illustrates the structures of fusion proteins used in this study. (B) Immunoblot analysis for endogenous CrkL and FA-CRKL proteins expressed in cells used in panel C. (C) Modified Boyden chamber haptotaxis assay. The number of cells (cells migrated/field) that had traversed to reach the lower surface coated with fibronectin through pores in the insert that separated two chambers was determined 3 h after plating cells into the upper chamber. Error bars indicate the standard deviation of the sample data obtained in four individual microscopic fields under a 40× objective lens. Similar results were obtained in three independent experiments.