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ICM time course and aza-dC dose response in different cell lines. (A and B) Time course of DNMT1 adduct formation. HeLa cells (A) or Jurkat cells (B) in exponential growth were treated with 5 μM aza-dC or untreated. Panel A shows the ICM slot blot for a single DNA concentration of 3 μg. Panel B shows the digitized data where the ICM signal was arbitrarily assigned unit values based upon phosphorimaging of a single DNA concentration on the ICM slot blot.
