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. 2003 Mar 24;5:78–89. doi: 10.1251/bpo49

Fig. 2.

Fig. 2

DNA/RNA cleavage by Fe-BABE conjugated w.t. and cys-minimized (7-) enzyme. Lanes 1-14: ECs halted at +13 (lanes 2, 4, 5, 7, 9, 11, 12, 14) or ITCs halted at +6 (lanes 3, 6, 10, 13) were formed with DNAs in which either the 5'-end of the T strand (lanes 2-7) or NT strand (lanes 9-14) was labeled with γ32P-ATP, and with either Fe-BABE conjugated w.t. (lanes 2-4, 9-11) or 7- (lanes 5-7, 12-14) enzyme H202 was added to lanes 2, 3, 6, 7, 10, 11, 13 and 14, and cleavage was mapped by reference to G+A ladders (lanes 1, 8). Cleavages are highlighted by vertical bars. Lanes 15-22: ITCs halted at +7 (lanes 15-18) or ECs halted at +24 (lanes 19-22) were formed with 3'-end labeled RNAs, and with either Fe-BABE conjugated w.t. (lanes 15, 16, 19, 20) or 7- (lanes 17, 18, 21, 22) enzyme. H202 was added to lanes 16, 18, 20, 22. Templates were a duplex T7 promoter from -17 to +25 in lanes 1-14,supercoiled pT7-7 plasmid in lanes 15-18 and a linearized plasmid (pPK7) containing a T7 promoter in lanes 19-22.