. 2003 Apr;185(8):2520–2527. doi: 10.1128/JB.185.8.2520-2527.2003

TABLE 2.

Alignment of putative C. cellulovorans promoters located at −10 and −35 bases upstream of the start point of transcription

Gene	Sequence alignment^a					Coordinate^c
Gene	Leader	−35	Gap (17 bp)	−10	TSP^b	Coordinate^c
cbpA	`tTTtTTaAAAaAcccAcc`	`TAaAAA`	`aaacgTtAtcaTCgAaT`	`TATtAT`	`AATGAcaAaTgAAAAAA`	−233
engE	`AaaaaTgAcATAtAtAtg`	`agGAAA`	`TTTAcagAAaTatAgta`	`TATAAT`	`tAaaAATgTgTAtgtAt`	−97
manA	`ATTtTgTAAAatAAggAA`	`TtGAtA`	`TTTAtTAAgtggatACg`	`aATAAg`	`gATGgagAgTTtAAcAc`	−64
hbpA	`AcTagaTAAATAAtAAAA`	`TAaAAA`	`cTgtagAAAgTTCAcCT`	`TtTtAc`	`AgTtttTATTctAAAgA`	−61
Consensus	`ATT-TTTAAATAAAAAAA`	`TAGAAA`	`TTTA-TAAA-TTCAACT`	`TATAAT`	`AATGAATATTTAAAAAA`

The consensus sequence derived from this alignment is given at the bottom. It consists of nucleotides that are present in any given position in more than 50% of the sequences. Promoter sequence nucleotides that match those of the consensus sequence are capitalized and in boldface; those that do not are lowercase and in lightface.

TSP, transcription start point (indicated by underline).

Nucleotide numbering begins from the first codon. The coordinates refer to the following published nucleotide sequences: cbpA (22), GenBank accession no. M73817; engE (27), GenBank accession no. AF105331; and manA and hbpA (25, 28), GenBank accession no. AF132735.