FIG. 3.
Expression of lacZ and LEU2 in strain EGY48[p8op-lacZ] carrying different pairs of the ClpQ, ClpY, or ClpYΔI fusion protein. The BD vector is pGilda, and the AD vector is pB42AD. Each lane represents the pairwise encoded fusion proteins from the BD or AD plasmid carrying fusion genes as indicated. A dash indicates that no proteins were fused to the BD or AD domain. In the column, each bar represents an average of the β-galactosidase levels with the standard deviation value in Miller units (18). Colony color was evaluated on Gal+Raf−Ura−His−Trp plates containing X-Gal over 2 days. The solid bar represents blue colonies; the shaded bar represents the light blue colonies. LEU2 expressions were tested on Gal+Raf−His−Trp−Leu plates over 4 days. +, Rapid growth of yeast cells in the selective medium as a result of elevated expression of the LEU2 gene; +/−, flash growth.