TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| R. leguminosarum bv. trifolii | ||
| RtTA1 | Wild type; Strr Rifr | 7 |
| RtAH1 | RtTA1 with pssT interrupted by integration of plasmid pAH1; Kmr | This work |
| E. coli | ||
| DH5α | supE44 ΔlacU169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 47 |
| ET8000 | rbs lacZ::IS1 gyrA hutCK | 23 |
| S17-1 | 294 derivative with RP4-2-Tc::Mu-Km::Tn7 chromosomally integrated | 48 |
| JM101 | supE thi Δ(lac-proAB) F′ (traD36 proAB+lacIqlacZΔM15) | 47 |
| Plasmids | ||
| pK19mobGII | pUC19 derivative, lacZα mobgusA; Kmr | 25 |
| M13mp19 | Phage sequencing vector | 47 |
| pSK4158 | ori P15A, promoterless phoA; Cmr | 43 |
| pUCphoA | Derivative of pUC19 and pSK4158; Apr | This work |
| pNM480 | ori ColE1, promoterless lacZY; Apr | 37 |
| pUC98 | pUC19 with 2,728-bp EcoRI-HindIII fragment of RtTA1 containing pssN and fragment of the pssT gene | 33 |
| pLT59 | M13mp19 with 1.9-kb KpnI fragment of RtTA1 containing an incomplete pssT gene | This work |
| pAH1 | pK19mobGII with 1,058-bp EcoRI/KpnI fragment of pLT59 | This work |
| pTA52PHO/LAC | This work | |
| pTV78PHO/LAC | This work | |
| pTA133PHO/LAC | This work | |
| pTA158PHO/LAC | This work | |
| pTA201PHO/LAC | This work | |
| pTA243PHO/LAC | This work | |
| pTA269PHO/LAC | This work | |
| pTA311PHO/LAC | This work | |
| pTA385PHO/LAC | This work | |
| pTL430PHO LAC | This work | |
| pTA455PHO/LAC | This work | |
| pTA480PHO/LAC | This work |
a
Strr, Streptomycin resistance; Rifr, rifampin resistance; Kmr, kanamycin resistance; Cmr, chloramphenicol resistance; Apr, ampicillin resistance; rbs, ribosome binding site; mob, mobility region.