TABLE 3.
AP and β-galactosidase activities for the PssT-PhoA and PssT-LacZ hybrid proteinsa
| Position of fusion in PssT | Mean activity (Miller units) ± SD of:
|
|
|---|---|---|
| PhoA | LacZ | |
| No fusionb | 95.66 ± 8 | 2.81 ± 0.29 |
| Ala-52 | 4,191.29 ± 491 | 4.45 ± 2.54 |
| Val-78 | 57.13 ± 17 | 46.08 ± 6.22 |
| Ala-133 | 3,651.29 ± 105 | 1.69 ± 0.92 |
| Ala-158 | 1,322.2 ± 646 | 52.61 ± 6.05 |
| Ala-201 | 6,573.19 ± 616 | 3.91 ± 0.45 |
| Ala-243 | 118.54 ± 52 | 42.81 ± 5.48 |
| Ala-269 | 3,256.6 ± 275 | 3.02 ± 0.72 |
| Ala-311 | 118.07 ± 40 | 58.62 ± 0.85 |
| Ala-385 | 717.48 ± 87 | 4.19 ± 3.82 |
| Leu-430 | 98.52 ± 5 | 55.90 ± 3.76 |
| Ala-455 | 651.05 ± 142 | 3.97 ± 0.04 |
| Ala-480 | 87.74 ± 9 | 1.94 ± 0.18 |
a
All assays were performed in E. coli strain ET8000 and are averages from at least three independent experiments. Values for PhoA are as defined by Manoil (30).
b
The PhoA and LacZ activities in ET8000 pUCphoA and ET8000 pNM480, respectively, are given.