FIG. 1.

Immunoprecipitation of p47 with MAb B3 and reactivity of rabbit antisera with p47. (A) M. gallisepticum strain ts-11 cell proteins radiolabeled with [³⁵S]methionine-cysteine and immunoprecipitated with MAb B3 (lane 1) or 71 (lane 2). The radiolabeled immunoprecipitated proteins, together with ¹⁴C-labeled standards (Amersham Pharmacia Biotech), were separated by SDS-12.5% PAGE. (B) Affinity-purified protein from a MAb B3 Sepharose column together with low-molecular-weight standards (Bio-Rad) separated by SDS-12.5% PAGE and stained with CBB. (C) Western blot of whole-cell proteins of M. gallisepticum strains ts-11 and S6. Cellular proteins and prestained molecular weight markers (New England BioLabs) were separated by SDS-12.5% PAGE, Western transferred, and immunostained with rabbit anti-p47 sera.