FIG. 3.

Pictorial representation of the cloned BglII DNA fragment encoding p47. The 3.894-kbp DNA sequence contained three ORFs, one complete ORF encoding p47 (ORF2) and two partial ORFs. The predicted leader sequences of ORF2 and -3 are boxed and hatched, and the acylated cysteine residue and the three amino acids immediately preceding each are also shown. The recognition sites for BglII, EcoRI, and HindIII are shown, together with the region identical to the sequence of oligonucleotide AZ (stippled box in ORF2). The positions of the UGA tryptophan codons are shown below ORF2 (p47). The positions for selected oligonucleotide primers from Table 1 are shown.