TABLE 1.
Oligonucleotides used in this study
| Oligo- nucleotide | Sequencea (5′-3′) |
|---|---|
| BQ | ggatccTCTAGTGGTGCTGCTTCTTCAC |
| BP | GTTCCAGGTCGTTTcCACACACCATTGTTAAC |
| BO | GTTAACAATGGTGTGTGgAAACGACCTGGAAC |
| BN | TGTTATCTGCTGCATTAGAGCTTACcCAAGAA |
| BL | TTGgGTAAGCTCTAATGCAG |
| BU | GTAGCTAAGTTATTcCATCCATATCCATAG |
| BT | CTATGGATATGGATGgAATAACTTAGCTAC |
| BG | gtcgacTTAGTTTAATTTCTTGAAGAC |
| BC | CTGTTGAGAAAGCGATTGTC |
| BD | CATATTCAGTGCTGTAGAAG |
| CB | gaattcCTAGTAGAGAAAACTGGAAA |
| BR | TAAGAAAAATGGAactagtATTTCAGCAAT |
| BS | ATTGCTGAAATactagtTCCATTTTTCTTA |
| CD | GAGTTGTATACCTCTATAGC |
| CE | GCATAGTCATTATATAATCCGAC |
| AZ | GATCAAACTACTCCAAGACAAGAAATGTCTGATC |
| TetFor | GAAAagatctGGAGTAATTGGAAG |
| TetRev | actagtCCATATTTATATAACAACTT |
a
Lowercase indicates nuclotide modification to produce a restriction endonuclease cleavage site or alter the UGA tryptophan codon to UGG.