FIG. 1.
Construction of the P. syringae pv. syringae B728a EEL deletion mutation and its corresponding complementation clone. (A) Physical map of the P. syringae pv. syringae B728a EEL. Pointed boxes indicate the predicted ORFs and the direction of transcription, with black triangles representing the presence of an Hrp box promoter. Hatched regions represent mobile genetic elements, and open boxes are putative effector or chaperone genes. (B) EEL deletion mutant CUCPB5111, with the dotted line representing the internal deletion that is replaced by an ΩSpr cassette. (C) The 10,488-bp XhoI fragment shown contains hrpK to tRNALeu in pCPP3092.