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Immunoblot analysis of HopPsyC-FLAG, HopPsyE-FLAG, and HopPsyV-FLAG secretion by E. coli carrying an intact (pCPP3042) or defective (pCPP3127) E. chrysanthemi type III secretion system. The supernatant fraction (S) was concentrated 7.5 times more than the cell-bound fraction (C). The upper panel was immunostained with anti-FLAG monoclonal antibody, and the bottom panel was stained with anti-β-galactosidase serum.
