TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| E. coli DH5αF′ | F−/endA1 hsdR17(rK− mK+) supE44 thi-1 recA1 gyrA (Nalr) relA1 Δ(laclZYA-argF)U169 deoR (φ80dlac Δ[lacZ]M15) | Life Sciences Technologies |
| TOP10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7697 galU galK rpsL(Strr) endA1 nupG | Invitrogen |
| P. syringae pv. syringae 61 | Wild type isolated from wheat, Nalr | 20 |
| pv. syringae 226 | Wild type, Nalr | T. Denny |
| pv. syringae B728a | Wild type, Rifr | 50 |
| pv. tabaci 11528 | Wild type, Rifr | American Type Culture Collection |
| pv. angulata Pa9 | Wild type | D. K. Willis |
| pv. atrofaciens B143 | Wild type | T. Denny |
| pv. delphinii PDDCC 529 | Wild type | T. Denny |
| pv. glycinea race 4 | Wild type | C. J. Baker |
| pv. morsprunorum PDDCC 5795 | Wild type | T. Denny |
| pv. mori PDDCC 4331 | Wild type | T. Denny |
| pv. phaseolicola B130 | Wild type | T. Denny |
| pv. tomato DC3000 | Wild type, Rifr | D. Cuppels |
| CUCPB5102 | B728a ΔhrcC::nptll, HR−, Rifr Kanr | 17 |
| CUCPB5111 | B728a ΔEEL::ΩSpr, HR+, Rifr Sptr | This work |
| pBluescript KS or SK | ColE1, Apr, MCS-lacZa | Stratagene |
| pCR-XL-TOPO | ColE1, MCS-lacZccdB, Kmr, Zeocinr | Invitrogen |
| pUCP24 | ColE1, ori1600, MCS-lacZ, Gmr | 48 |
| pFLAG-CTC | For construction of C-terminal fusion to FLAG peptide, Apr | Sigma |
| pRK415 | Broad-host-range vector unstable in absence of selection, Tcr | 23 |
| pRK2013 | IncP Tra RK2+ ΔrepE1+ Kmr | 12 |
| pCPP46 | IncP LacZ′ Par+; Tcr | D. W. Bauer |
| pCPP3042 | pCPP46 carrying the E. chrysanthemi AC4150 hrp/hrc genes, Tcr | This work |
| pCPP3127 | pCPP3042 with a mutation in hrcT (hrcT::ΩSpr), Tcr Spr | This work |
| pCPP3092 | pCPP46 carrying the P. syringae B728a hrpK and EEL, Tcr | This work |
| pCPP3105 | pCR-XL-TOPO::pathovar tabaci EEL, Kmr, Zeocinr | This work |
| pCPP3106 | pCR-XL-TOPO::pathovar glycinea EEL, Kmr, Zeocinr | This work |
| pCPP3107 | pCR-XL-TOPO::pathovar angulata EEL, Kmr, Zeocinr | This work |
| pCPP3108 | pCR-XL-TOPO::pathovar phaseolicola EEL, Kmr, Zeocinr | This work |
| pCPP3109 | pCR-XL-TOPO::pathovar syringae 226 EEL, Kmr, Zeocinr | This work |
| pCPP3110 | pCR-XL-TOPO::pathovar tomato DC3000 EEL, Kmr, Zeocinr | This work |
| pCPP3111 | pCR-XL-TOPO::pathovar syringae B728a EEL, Kmr, Zeocinr | This work |
| pCPP3113 | pCR-XL-TOPO::pathovar morsprunorum EEL, Kmr, Zeocinr | This work |
| pCPP3115 | pCR-XL-TOPO::pathovar delphinii EEL, Kmr, Zeocinr | This work |
| pCPP3116 | pCR-XL-TOPO::pathovar atrofaciens EEL, Kmr, Zeocinr | This work |
| pCPP3117 | pFLAG-CTC::hopPsyC, Apr | This work |
| pCPP3118 | pFLAG-CTC::ORF6-hopPsyV, Apr | This work |
| pCPP3119 | pFLAG-CTC::hopPsyE, Apr | This work |
| pCPP3153 | pUCP24::hopPsyC-FLAG, Gmr | This work |
| pCPP3154 | pUCP24::hopPsyE-FLAG, Gmr | This work |
| pCPP3155 | pUCP24::ORF6-hopPsyV-FLAG, Gmr | This work |
a
MCS, multiple cloning site.