TABLE 3.
P. syringae strains and primers used to isolate the EELs by PCR
| P. syringae pathovar or strain | Primers | Plasmid | Amplified fragment length (bp) | Encoded proteins and homologsa (% identity) | GenBank accession no. |
|---|---|---|---|---|---|
| pv. mori PDDCC 4331 | P580, P706 | pCPP3114 | 2,249 | AvrPphEb | AF461553 |
| pv. angulata Pa9 | P602, P577 | pCPP3107 | 2,731 | AvrPphE (97.9)d | AF461554 |
| pv. atrofaciens B143 | P580, P706 | 2,275 | ShcA (55)c; HopPsyA (31)c | AF461555 | |
| pv. delphinii PDDCC 529 | P580, P706 | pCPP3115 | 4,599 | AvrPphE (73.4)d; ORF2 (86)i; AvrPphF1 (50)e; AvrPphF2 (52)f | AF461556 |
| pv. glycinea race 4 | P579, P577 | pCPP3106 | 2,878 | AvrPphE (99.5)d | AF461557 |
| pv. morsprunorum PDDCC 5795 | P580, P706 | pCPP3113 | 2,277 | ShcA (56)c; HopPsyAb | AF461558 |
| pv. phaseolicola B130 | P579, P578 | pCPP3108 | 2,561 | AvrPphE (100)d | AF461559 |
| pv. syringae 226 | P579, P578 | pCPP3109 | 3,100 | ShcA (100)c; HopPsyA (88.6)c | AF461561 |
| pv. syringae B728a | P579, P577 | pCPP3111 | 8,286 | HopPsyC (38)g; HopPsyE (77.1)d; HopPsyV (21)h | AF232005 |
| pv. tabaci 11528 | P579, P577 | pCPP3105 | 2,782 | AvrPphE (98.9)d | AF461560 |
| pv. tomato DC3000 | P602, P577 | pCPP3110 | 6,816 | ORF1; ORF2; ORF3; ORF4 | AF232004 |
a
GenBank accession numbers of homologous proteins are given below.
b
The predicted ORF is smaller than published sequences due to nonsense mutations in the coding region.
c
Strain 61 ORF1 and HopPsyA, AF232003.
d
pv. phaseolicola 1302A AvrPphE, U16817.
e
pv. phaseolicola AvrPphF1, AAF67151.
f
pv. phaseolicola AvrPphF2, AAF67152.
g
pv. phaseolicola AvrPphC, AAA83419.
h
pv. versicatoria AvrBs T, AF156163.
i
pv. tomato DC3000 ORF2, AF232004.