TABLE 2.
Affinities for interactions between homogenous domains of TolA and between different domains of TolA, measured by BIAcore analysis
| Interactiona | kab (M−1 s−1) | kdb (s−1) | KA (M−1) | KD (μM) |
|---|---|---|---|---|
| TolA-TolA | (3.4 ± 1) × 105 | 0.12 ± 0.008 | 2.8 × 106 | 0.4 |
| TolA-TolAI | (5.3 ± 1.1) × 105 | 0.12 ± 0.01 | 4.5 × 106 | 0.2 |
| TolA-TolAII | (2.3 ± 0.2) × 103 | (9.3 ± 0.4) × 10−3 | 2.5 × 105 | 4 |
| TolA-TolAIII | (7.0 ± 0.5) × 103 | (69 ± 3) × 10−3 | 1.0 × 105 | 9.9 |
| TolAII-TolAII | (7.4 ± 1) × 103 | (54 ± 4) × 10−3 | 1.4 × 105 | 7.2 |
| TolAIII-TolAI | (9.4 ± 0.9) × 103 | (5.7 ± 0.4) × 10−3 | 1.6 × 106 | 0.6 |
| TolAIII-TolAII | (1.5 ± 0.08) × 103 | (4.4 ± 0.1) × 10−3 | 3.4 × 105 | 2.9 |
| TolAIII-TolAIII | (5.1 ± 0.5) × 103 | (24 ± 1.3) × 10−3 | 2.1 × 105 | 4.7 |
a
The ligand is given first in each entry.
b
Data are means ± standard deviations.