FIG. 3.

Multiple sequence alignment of the region encompassing the amidase signature sequence (boldface) of enzymes identified as having sequence homology to MHA. The enzymes are in decreasing order of similarity to MAH and were identified by a BLAST search (1) of the nonredundant protein database. The top-scoring example of each enzyme was used for this alignment. MAH, MAH from P. putida; IAAH, indoleacetamide hydrolase from A. rhizogenes (gi:1170450), GATA, glutamyl-tRNA amidotransferase subunit A from S. coelicolor (gi:6225417); ESAM, enantiomer-specific amidase from Rhodococcus (gi:98837); AMID, amidase from R. erythropolis (gi:113712); PYRA, pyrazinamidase/nicotinamidase from Caulobacter crescentus (gi:13424188); UREA, urea amidolyase from S. cerevisiae (gi:6319685); ACET, acetamidase from Aspergillus ustus (gi:13569683); 6AHH, 6-aminohexanoate-cyclic-dimer hydrolase from Flavobacterium sp. strain K172 (gi:129000); MAE2, malonamidase E2 from B. japonicum (gi:17939381); FAAH, FAAH from Homo sapiens (gi:6225310); VD3H, vitamin D₃ hydroxylase-associated protein from Gallus gallus (gi:2492838).