TABLE 2.

MAH activity in crude cell extracts^a

P. putida strain	Carbon source	MAH activity (nmol/min/mg)b
ATCC 12633	d-Glucose	15.6 ± 1.2
ATCC 12633	R,S-Mandelate	31.0 ± 1.1
ATCC 12633	R,S-Mandelamide	103 ± 3.0
ATCC 12633	R,S-Mandelonitrile	—c
ATCC 17453	d-Glucose	NDd
ATCC 17453	R,S-Mandelamide	—c
ATCC 17453 (pSCR4)	R,S-Mandelamide	113 ± 5.2

^aCells were grown on M9 minimal medium containing either d-glucose, R,S-mandelate, or R,S-mandelamide (20 mM).

^bAssays were carried out at 30°C and pH 7.8 as described in Materials and Methods. The data are the mean ± standard deviation of at least three individual experiments.

^c—, strain unable to grow.

^dND, no activity detected.