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Kinetic parameters for BADHa
| Cofactorb | Km for benzalde- hyde (μM) | kcat (min−1) | kcat/Km (min−1 M−1) |
|---|---|---|---|
| NAD+ | 63.4 ± 1.5 | (8.24 ± 0.32) × 103 | 1.3 × 108 |
| NADP+ | 39.9 ± 4.4 | (2.55 ± 0.10) × 103 | 6.39 × 107 |
Experiments were carried out at 25°C and pH 8.5 as described in Materials and Methods. The data are the mean ± standard deviation of at least three individual experiments.
Cofactor concentrations were maintained at 1 mM.
