FIG. 2.

Actin and ezrin recruitment visualized by confocal examination of infected monolayers of HUVECs. HUVECs were infected with either wild-type bacteria (Z5463) or mutants. Experiments were carried out after 4 h of infection (localized adhesion). The localization of bacterial colonies is shown in the first column by phase contrast. F-actin was labeled with a phalloidin probe (second column) and with ezrin by using a polyclonal antibody (third column). Results obtained with the wild-type strain and three of the mutants are shown. Similar results were obtained with the remaining seven mutants.