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. 2003 Apr;185(8):2432–2440. doi: 10.1128/JB.185.8.2432-2440.2003

FIG. 4.

FIG. 4.

CpxP is not a negative regulator under stress conditions. Western blot analysis was performed on PAD726 (lanes 1 and 2), PAD727 (lanes 3 and 4), and PAD728 (lanes 5 and 6) following growth in sodium phosphate-buffered LB broth at pH 5.5 (lanes 1, 3, and 5) or 8.5 (lanes 2, 4, and 6) as described in Materials and Methods. Under noninducing conditions, both MBP-CpxP and MBP-CpxPA24D negatively regulate the Cpx pathway (compare the β-galactosidase level in lane 1 to those in lanes 3 and 5). However, upon induction by alkaline pH, β-galactosidase levels increase in the presence or absence of MBP-CpxP or MBP-CpxPA24D (compare lane 2 to lanes 4 and 6).