TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype | Strain construction | Source or reference |
|---|---|---|---|
| Strains | |||
| MC4100 | F−araD139 Δ(argF-lac) U169 rpsL150 (Strr) relA1 flbB5301 deoC1 ptsF25 rbsR | 36 | |
| TR50 | λRS88 [cpxP′-lacZ+] | 34 | |
| PAD282 | MC4100 λRS88 [cpxP′-lacZ+] | MC4100 × TR50 | This study |
| PAD501 | PAD282(pHJ8) | This study | |
| PAD726 | PAD282(pMal-p2) | This study | |
| PAD727 | PAD282(pMCP) | This study | |
| PAD728 | PAD282(pMCPss) | This study | |
| TR70 | cpxR1::spc | 31 | |
| PAD292 | PAD282 cpxR1::spc | PAD282 × TR70 | This study |
| PAD81 | cpxRM199A zii::Tn10 | This study | |
| PAD293 | PAD282 cpxRM199A zii::Tn10 | PAD282 × PAD81 | This study |
| WBS262 | nlpE::spc | 39 | |
| PAD485 | PAD282 nlpE::spc | PAD282 × WBS262 | This study |
| PAD486 | PAD485(pHJ8) | This study | |
| CLC393 | cpxP::kan | Laboratory stock | |
| PAD488 | PAD282 cpxP::kan | PAD282 × CLC393 | This study |
| TR212 | cpxA24 zii::Tn10 | Laboratory stock | |
| PAD455 | PAD282 cpxA24 zii::Tn10 | PAD282 × TR212 | This study |
| PAD504 | PAD455(pHJ8) | This study | |
| TR235 | λRS88 [cpxR′-lacZ+] | 32 | |
| PAD279 | MC4100 λRS88 [cpxR′-lacZ+] | MC4100 × TR235 | This study |
| PAD290 | PAD279 cpxRM199A zii::Tn10 | This study | |
| TR49 | λRS88 [degP′-lacZ+] | 34 | |
| PAD280 | MC4100 λRS88 [degP′-lacZ+] | This study | |
| SP969 | λ RS88 [yihE-dsbA′-lacZ+] | 6 | |
| PAD281 | MC4100 λRS88 [yihE-dsbA′-lacZ+] | This study | |
| TR530 | λRS88 [spy′-lacZ+] | 31 | |
| PAD283 | MC4100 λRS88 [spy′-lacZ+] | This study | |
| PAD593 | MC4100 λRS88 [ppiA′-lacZ+] | This study | |
| Plasmids | |||
| pMal-p2 | Cloning vector for making periplasmic MBP fusion proteins; parent of pMCP and pMCPss | New England Biolabs | |
| pMCP | Overexpression plasmid for periplasmic MBP-CpxP fusion protein | 31 | |
| pMCPss | Overexpression plasmid for tethered MBP-CpxPA24D fusion protein | 31 | |
| pHJ8 | IPTG-inducible PapG expression plasmid | 20 | |
| pGS284 | Cloning vector for allelic exchange; parent of pCpxRM199A and pCpxRD51A | 38 | |
| pCpxRM199A | Plasmid used for recombination of cpxRM199A at the cpxR locus | This study | |
| pCpxRD51A | Plasmid used for recombination of cpxRD51A at the cpxR locus | This study |