TABLE 2.
Primers used in this study
| Primer name | Primer sequencea |
|---|---|
| SFLGG-NdeA | GGC ATA TGA TGA TCA GGT CAT TAT GGA |
| SFLGG-BamB2 | GCC GGT GGC GGA TCC CCC ACC GGC |
| SFLGJ-NdeA1 | GGA AAT CAT CAT ATG ATC GGA GAC |
| SFLGJ-BamB1 | AGC GAC TAC GGA TCC TTG AGC AAT |
| SFLIE-NdeA1 | CAG GGG AAT CAT ATG GCA GCA ATA |
| SFLIE-BamB1 | GTG TTG TCA GGA TCC GCT TAA ACC |
| SFLGB-NdeA1 | TGC GGA GGA CAT ATG CTC GAC AGG |
| SFLGB-BamB2 | TAA CAG CGC GGA TCCTTA GTT TCC |
| SFLGC-NdeA1 | GGA AAC TAA CAT ATG GCG CTG TTA |
| SFLGC-BamB1 | GAC ATA CGC GGA TCC TTT ACT GGC |
| SFLGF-NdeA1 | CGG GAT AGC CAT ATG GAT CGC GCA |
| SFLGF-BamB1 | AAA ATG TGG ATC CTT AAC TCA TCG |
| SFLGD-NdeA | AAG GAG GCG CAT ATG TCT ATT GCC |
| SFLGD-BamB | CTG ACT CCT GGA TCC TGT AAG GGC |
| SFLGL-NdeA | GGA GAA GGA TCA TAT GCG TAT CAG |
| SFLGL-BamB | TTC GTG ATA GGA TCC AAA AAG AGG |
a
Restriction sites are indicated by underlining; start and stop codons (where present on the primer) are indicated by italics.