TABLE 1.
Strains and plasmids used in this work
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| Strains | ||
| DJ901 | Δ(soxRS) derivative of GC4468 | 17 |
| EH46 | DJ901 lysogenized with λ(soxS promoter-lacZ) | 22 |
| EH86 | DJ901 lysogenized with λ(16-bp spacer mutant soxS promoter-lacZ) | 22 |
| EH200 | DJ901 lysogenized with λ(soxR promoter-lacZ) | 24 |
| GC4468 | K12 rpsL thi soxR+soxS+ | 17 |
| MC9 | EH200 with chromosomally integrated 2.8-kb fusion fragment cat-120-bp soxS promoter-sacB in the araD site | This work |
| NR9273 | ara thi Δ(pro-lac) mutD5 zaf-13::Tn10 | R. Schaaper |
| TN402 | GC4468 soxR::kan | T. Nunoshiba |
| Plasmids | ||
| pACYC184 | Low-copy-number cloning vector (Cmr) | New England Biolabs |
| pBD100 | pBR322 with entire soxRS locus (Ampr) | 1 |
| pKO3 | Plasmid with B. subtilis sacB gene (Cmr) | 27 |
| pMC6 | pACYC184 with cat-soxS promoter-sacB (Cmr) | This work |
| pSE380 | trc promoter-containing plasmid with lacIq gene (Ampr) | Invitrogen |
| pSXR | pSE380 derivative containing the wild-type soxR allele (Ampr) | 1 |