TABLE 3.
Maximum germination rates of spores of various strains as a function of the concentration of AGFKa
| Spore genotype (strain) | Germination rate (% maximum loss in OD600/h) at indicated AGFK concnb
|
||||
|---|---|---|---|---|---|
| 1n | 3n | 9n | 27n | 81n | |
| Wild-type (PS832) | 3 | 16 | 90 | 162 | 205 |
| PsspD::gerA (PS3476) | 3 | 7 | 45 | 74 | 105 |
| PsspD::gerB (PS3477) | 3 | 12 | 105 | 180 | 232 |
| PsspB::gerB (FB58) | 2 | 14 | 89 | 155 | 210 |
| PsspD::gerK (PS3478) | 3 | 12 | 78 | 160 | 200 |
a
Spores of various B. subtilis strains were germinated at 37°C in 50 mM KPO4 (pH 7.4) with various concentrations of asparagine, glucose, and fructose (note that K+ levels were constant), the OD600s were measured, and the maximum germination rates were determined as described in Materials and Methods.
b
The concentrations of asparagine, glucose, and fructose represented by n are 31 μM, 6 μg/ml, and 6 μg/ml, respectively.