TABLE 6.
Effect of deletion of gerK or overexpression of gerA on spore germination due to the GerB* receptora
| Spore genotype (strain) | Germination rate (% maximum loss in OD600/h) at the indicated l-asparagine concn
|
||
|---|---|---|---|
| 330 μM | 1 mM | 3 mM | |
| gerB* (FB10) | 30 | 57 | 78 |
| gerB* ΔgerK (PS3498) | 19 | 42 | 52 |
| PsspB::gerB* (PS3415) | 145 | 270 | 294 |
| PsspB::gerK* ΔgerK (PS3503) | 67 | 153 | 205 |
| PsspD::gerA gerB* (PS3501) | 19 | ||
a
Spores of various B. subtilis strains were germinated at 37°C in 25 mM Tris-HCl (pH 8.2) and various concentrations of l-asparagine, and maximum germination rates were determined as described in Materials and Methods.