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PCR fragments of SRSR-2p and SRSR-1p were amplified from pNOB8, and SRSR-2c was amplified from the S. solfataricus P2 chromosome. Sequence identities between the repeats are indicated by boldface letters. Spacer sequences can be obtained from the sequence accession numbers given in Materials and Methods. Biotin is attached to the 5′ end of the DNA strand carrying the sequences given for SRSR-2p and SRSR-1p in order to facilitate protein purification.
