TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| S. aureus | ||
| RN4220 | Highly transformable strain; restriction deficient | 28 |
| RN6390 | Parental laboratory strain | 37 |
| KB350 | RN6390 cidA::Erm; Emr | This study |
| E. coli DH5α | Host strain for construction of recombinant plasmids | 22 |
| Plasmids | ||
| pDG647 | Source of Emr cassette; Emr Apr | 21 |
| pCL52.2 | Temperature-sensitive shuttle vector; Tcr Spr | 45 |
| pRN5548 | Gram-positive bacterial expression vector; Cmr | 35 |
| pRN-cidA | CidA ORF cloned into EcoRI and BamHI sites of pRN5548; Cmr | This study |