Figure 1.
Effect of IL-1β and CDCA on mRNA expression in human primary hepatocytes. Human primary hepatocytes were treated with IL-1β (10 ng/ml) or CDCA (50 μM) for time periods indicated. Quantitative Real time PCR was performed as described in Materials and Methods. The relative mRNA levels of CYP7A1, HNF4α, SHP and c-Jun were calculated with respect to the UBC mRNA. The mRNA expression at 0 hr (un-treated) was set as 1. The error bars represent the standard deviation of from three different experiments. N=3.