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. Author manuscript; available in PMC: 2007 Jun 1.
Published in final edited form as: Hepatology. 2006 Jun;43(6):1202–1210. doi: 10.1002/hep.21183

Figure 7.

Figure 7

Chromatin immunoprecipitation assay of HNF4α, PGC-1α, and c-Jun binding to the CYP7A1 chromatin. A. Anti-HNF4α antibody was used to precipitate chromatin from HepG2 cells treated with IL-1β (10 ng/ml) or CDCA (50 μM). B. Anti-c-Jun antibody was used to immunoprecipitate chromatin. C. HepG2 cells were transfected with cJun or JNK1 expression plasmid or pcDNA3 empty vector, and anti-HNF4α antibody was used to immunoprecipitate chromatin. D. HepG2 cells were transfected with HA-PGC-1α, and co-transfected with a c-Jun expression plasmid or pcDNA3 empty vector. A 391 bp fragment containing the BAREI and BAREII regions of the CYP7A1 promoter was PCR amplified and analyzed on a 1.5% agarose gel.