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. 2005 Aug 14;2005(3):131–138. doi: 10.1155/MI.2005.131

Figure 1.

Figure 1

IL-1β release induced by DNFB in a fetal skin dendritic cell line. Cells were stimulated with 2,4-dinitrofluorobenzene (DNFB, 1 μg/mL) or with 2,4-dichloronitrobenzene (DCNB, 1 μg/mL), for 30 minutes. Alternatively, cells were stimulated with GM-CSF (10 ng/mL) alone, or in association with DNFB, for the same time period. As a positive control, the cells were stimulated with LPS (40 μg/mL), for 12 hours. IL-1β was quantified with an ELISA kit. Data express the IL-1β release above the basal secretion. Each value represents the mean ± SEM from the indicated number of experiments, performed in duplicate. Statistical significance was calculated by the one-way ANOVA test with a Bonferroni post-test (∗∗∗ P < .001 as compared to the control; ⩲⩲⩲ P < .001 as compared to stimulation with DNFB).