Figure 3.

Transcriptional activity of Ogre retrotransposons in V. pannonica analyzed by RT–PCR. The reactions were performed on total RNA isolated from roots, leaves, or flowers (RT+). Negative controls with omitted reverse transcriptase step were included to check for false-positive results (RT−). (A) Amplification of the ORF1 sequence using primers VP17 and VP18. (B) Amplification of the region containing putative intron using primers VP25 and MT3.