Figure 1.

Western blot analysis of expression of zipper and l(2)gl in testes of 3-day-old Drosphila. (A) l(2)gl and zipper expression in D. simulans (Riverside) infected (DSR) and uninfected with Wolbachia (DSRT). (B) Effect of heat treatment (indicated +/−) on expression of l(2)gl and zipper in transgenic D. melanogaster carrying a construct with the indicated gene linked to a heat-shock promoter. Band intensity was quantified using AIDA software (Raytest). Values were normalized using α-tubulin as an internal control for equal loading (not shown). Bars show normalized mean band intensity values (± 1 SE). Sample size (n) is shown below each bar. The bands shown represent typical results.