Figure 3.

Expression of CI in hs-zip flies and hs-l(2)gl flies. Confocal images of fertilized eggs visualized by indirect immunofluorescence using an anti-sperm tail primary antibody (A, C, G), a DNA-specific green dye (A–C), propidium iodide (D–F and H), and an anti-α-tubulin antibody (green) (E and H). (A and B) Control crosses displaying normal egg development when fertilized by (A) non-heat-treated males (arrow, sperm tail) or (B) wild-type males. (C–F) Fertilization defects observed in crosses using heat-treated hs-zip males. (G and H) Typical examples of the early lethal phenotype observed in an incompatible cross between a heat-treated hs-lgl male and a wild-type female (H).