TABLE 1.

Effect of Zipper and Lgl on CI expression

Cross	Treatment	Eggs	Females	Proportion unhatched	Comparison
1	hszipper heat	2912	22	0.75 ± 0.05	1 vs. 2 (P < 0.01)
2	hszipper no heat	2654	17	0.22 ± 0.07
3	wt heat	1812	10	0.14 ± 0.047	1 vs. 3 (P < 0.01)
4	wt no heat	2199	15	0.19 ± 0.034	3 vs. 4 (NS)
5	hslgl heat	2356	31	0.66 ± 0.15	5 vs. 6 (P < 0.01)
6	hslgl no heat	2100	25	0.1 ± 0.063
7	wt heat	4988	29	0.26 ± 0.04	5 vs. 7 (P < 0.01)
9	wt no heat	3666	18	0.22 ± 0.077	7 vs. 9 (NS)

Incompatibility in single pair crosses between treated males (treatment) and non-heat-treated 3-day-old virgin hszipper and hslgl males and Wolbachia-free wild-type virgin females aged 2–5 days old. Crosses in which no eggs hatched were assumed to be due to the females being unfertilized and were not included in the analysis. Eggs were removed, counted daily, and then scored for hatching 12–24 hrs later. The proportion of eggs hatching was scored over 4 days. NS, not significant; wt, wild type.