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. 2006 Jun;173(2):599–610. doi: 10.1534/genetics.105.054783

Figure 4.

Figure 4.

Sir2, but not Hir1 or Bdf1, is required for the enhanced silencing due to Asf1 mutants. (A) Hir1 is only partially required for enhanced silencing by the 185T and 152I Asf1 mutants. All strains except WT have CAC1 deleted. Yeast strains ROY1172 with the pRS314 empty vector (WT), BAT034 with pAsf1-185T (hir1Δ Asf1-185T), BAT034 with pAsf1-152I (hir1Δ Asf1-152I), ROY1169 with pAsf1-185T (Asf1-185T), ROY1169 with pAsf1-152I (Asf1-152I), BAT034 with pAsf1 (hir1Δ), and ROY1171 with empty vector (vector) were plated in 10-fold serial dilutions onto media with 75% less adenine (low ade) or 1.0 mg/ml of 5′FOA. (B) Bdf1 is not required for enhanced silencing by the 185T and 152I Asf1 mutants. All strains except WT have CAC1 deleted. Yeast strains ROY1172 with the pRS314 empty vector (WT), BAT052 with pAsf1-185T (bdf1Δ Asf1-185T), BAT052 with pAsf1-152I (bdf1Δ Asf1-152I), ROY1169 with pAsf1-185T (Asf1-185T), ROY1169 with pAsf1-152I (Asf1-152I), BAT052 with pAsf1 (bdf1Δ), and ROY1171 with empty vector (vector) were plated in 10-fold serial dilutions onto media with 75% less adenine (low ade) or 1.0 mg/ml of 5′FOA. (C) Sir2 is required for enhanced silencing by the 185T and 152I Asf1 mutants. All strains except WT have CAC1 deleted. Yeast strains ROY1172 with the pRS314 empty vector (WT), BAT053 with pAsf1-185T (sir2Δ Asf1-185T), BAT053 with pAsf1-152I (sir2Δ Asf1-152I), ROY1169 with pAsf1-185T (Asf1-185T), ROY1169 with pAsf1-152I (Asf1-152I), BAT053 with pAsf1 (sir2Δ), and ROY1171 with empty vector (vector) were plated in 10-fold serial dilutions onto media with low adenine or 1.0 mg/ml 5′FOA.