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. 2006 Feb 16;8(2):R47. doi: 10.1186/ar1903

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Copyright © 2006 Lindberg et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Experimental design. (a) Orthopedic samples. Three orthopedic biopsies (b1, b2, and b3) were taken from each of seven patients at random sites of the synovium. For patients 1 to 3 each biopsy was split into three parts. RNA from each sub-biopsy was hybridized in duplicate (b1.AA and b1.AB refer to biopsy 1, sub-biopsy A, technical replicates A and B, respectively) versus the reference. The average weight of the sub-biopsies was 33 mg. For patients 4 to 7, RNA from each biopsy was hybridized in duplicate (b1.A and b1.B: biopsy 1, technical replicates A and B, respectively) versus the reference. The average weight of the biopsies from patients 4 to 7 was 29 mg. (b) Arthroscopic biopsies. Two biopsies (b1 and b2) were taken from each of patients 8 and 11 to 13, and four from each of patients 9 and 10. Each biopsy was hybridized in duplicate versus the reference. The average weight of the arthroscopic biopsies was 19 mg.