Table 2.
Adenosine concentration in peritoneal exudates
| Wild-type mice (nM ± SEM) | A2A knockout mice (nM ± SEM) | A3 knockout mice (nM ± SEM) | |
| Control | 118 ± 6 (n = 19) | 110 ± 6 (n = 14) | 133 ± 6 (n = 12) |
| Methotrexate (0.75 mg/kg/week) | 178 ± 12* (n = 15) | 162 ± 7** (n = 7) | 214 ± 10† (n = 9) |
Wild-type mice, A2A receptor knockout mice or A3 receptor knockout mice were treated with either weekly injections of methotrexate (0.75 mg/kg) or saline control for 4 weeks prior to induction of inflammation. Inflammatory exudates were induced in the peritoneum of mice, as described. After 4 hours the exudates were collected and the adenosine levels quantitated. Wild-type data are a combination from both mouse strains. *P < 0.0001 vs wild-type control mice, Student's t test; **P < 0.0001 vs A2A knockout control mice, Student's t test; † P < 0.0001 vs A3 knockout control mice, Student's t test.