Table 3.
Tumor necrosis factor alpha concentration in peritoneal exudates
| Wild-type mice (pg/ml ± SEM) | A2A knockout mice (pg/ml ± SEM) | A3 knockout mice (pg/ml ± SEM) | |
| Control | 42 ± 7 (n = 14) | 36 ± 8 (n = 10) | 75 ± 18* (n = 6) |
| Methotrexate (0.75 mg/kg/week) | 14 ± 4** (n = 15) | 25 ± 7 (n = 9) | 31 ± 11† (n = 8) |
Wild-type mice, A2A receptor knockout mice or A3 receptor knockout mice were treated with either weekly injections of methotrexate (0.75 mg/kg) or saline control for 4 weeks prior to induction of inflammation. Inflammatory exudates were induced in the peritoneum of mice, as described. After 4 hours the exudates were collected, centrifuged at 100 × g and frozen. Tumor necrosis factor alpha levels were later quantitated by ELISA. Wild-type data are a combination from both mouse strains. **P < 0.001 vs wild-type control mice, Student's t test; *P < 0.05 vs wild-type control mice, Student's t test; †P < 0.05 vs A3 knockout control mice, Student's t test.