Table 4.
IL-10 concentration in peritoneal exudates
| Wild-type mice (pg/ml ± SEM) | A2A knockout mice (pg/ml ± SEM) | A3 knockout mice (pg/ml ± SEM) | |
| Control | 62 ± 7 (n = 24) | 73 ± 9 (n = 12) | 115 ± 14** (n = 15) |
| Methotrexate (0.75 mg/kg/week) | 97 ± 18* (n = 12) | 41 ± 6† (n = 7) | 150 ± 31 (n = 7) |
Wild-type mice, A2A receptor knockout mice or A3 receptor knockout mice were treated with either weekly injections of methotrexate (0.75 mg/kg) or saline control for 4 weeks prior to induction of inflammation. Inflammatory exudates were induced in the peritoneum of mice, as described. After 4 hours the exudates were collected, centrifuged at 100 × g and frozen. IL-10 levels were later quantitated by ELISA. Wild-type data are a combination from both mouse strains. ** P < 0.001 vs wild-type control mice, Student's t test; * P < 0.05 vs wild-type control mice, Student's t test; †P < 0.05 vs A2A knockout control mice, Student's t test.