TABLE 2.
Primer sequences and optimal PCR conditions for 10 X chromosome microsatellites
| Locus name | Approximate chromosome position (Mb) | Accession no. | UniSTS | Primers (5′–3′) | Amplicon size | PCR [MgCl2] | TA PCR |
|---|---|---|---|---|---|---|---|
| BMS1616 | 7 | G18677 | 27189 | CAGTGTGTATAGCATGATTCCGAGTTGGTCTGCATTCATACATTAA | 92–116 | 1.25 | 54°–55° |
| BL1045 | 9.2 | 251338 | TGCCAGAACAAGTCACAAGCCTCCAAGGGTGTCTCTATCCC | 92–100 | 1.5 | 59° | |
| UWCA19 | 9.4 | U01794 | 251002 | CTGTGATAACCTATATGGGAAAGGAGAGGACAGCAAAGTGATTCAGT | 97–105 | 1.5 | 58° |
| BL1098 | 10 | 251324 | CCACAACTTCCAGAAGCCTCCAGAAACCACCCAAACTAACC | 203–235 | 1.25 | 56° | |
| BMS960 | 11 | G18760 | 74452 | TGTAAATAGCTCCCCTCCTGCCTAGCAATTTGTTGTTCATGGC | 106–128 | 1.75 | 57° |
| XBM701 | 11.7 | 251432 | TTCCCCTTGAAATCATCTGGTCCAAGTTACCAAAATTGACCC | 148–168 | 2.0 | 58° | |
| BMS2713 | 12.2 | G18462 | 69721 | TGAATACCTGTTTCCAGCCCCTCCTAAGTCCAGGAAGCCC | 143–155 | 1.5 | 56° |
| XBM7 | 251010 | CTGTATTAGAGTTCCCTGGAGAAAGCCAACATGCCCTGTAGAAT | 180–202 | 1.5 | 57° | ||
| XBM361 | 14.8 | 251341 | ACACACTGAGAATTAAACTACAAAAACCACACAACTGAAGCCACTTTAAGC | 150–162 | 1.25 | 58°–59° | |
| BMS649 | 16.3 | G18728 | 37262 | CTCGGACTCATAACGCACATCGAGCAACAAGAGTGAAGGT | 116–134 | 1.75 | 59° |
Magnesium chloride concentration is shown by [MgCl2] and annealing temperature is denoted by TA. The UniSTS column gives accession numbers for the database of sequence tagged sites (http://www.ncbi.nlm.nih.gov/genome/sts).