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H3K9me2 and H3K27me2 staining do not overlap. (A) Colabeling with anti-H3K9me2 and anti-H3K27me2 reveals no significant overlap. (B) Colabeling control showing that two different antibodies against H3K27me2 (Upstate 07-452 from rabbit and Abcam 14222 from rat, labeled H3K27me2_a and H3K27me2_b) when applied together label the same regions of pachytene chromosomes. Minor nonconcordance can be attributed to chromatic aberration.
