Figure 4.—

Comparative gene organization for (A) Dbp80 and (B) RpL15 in six Drosophila species and An. gambiae. cDNA sequences were available for D. melanogaster, D. yakuba (DyRpL15 only), D. ananassae (5′ EST for DaDbp80 only), D. pseudoobscura, D. virilis, and An. gambiae, and these cDNA sequences were used in alignment to resolve the gene structures from available genomic sequence for the cognate species. Where cDNA sequence was not available, the most closely related cDNA sequence was used (for example, the D. virilis cDNA was used to resolve the D. mojavensis gene structure, etc.) The sequence of the second exon of Dbp80 is not well conserved across species in the Sophophoran sublineage and therefore not detectable in the current assemblies of the D. yakuba and D. Willistoni genomes.